Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. 5 hours. 1700 +1 800. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. VIS absorbance detection. Turn on Plate Reader and wait for it to complete its start-up routine. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The opaque walls prevent stray light from being detected from neighbouring. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. White plates reflect the light, enabling even low levels of Luminescence to be detected. Integrated to. . The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Every step is optimized to simplify analysis and reporting to support our microplate readers. SpectraMax i3x. Timothy Bolus, MPM, MBA, Compliance Program Manager. Cellular. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. ELISA Plate Readers / Microplate Readers. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 0%, raw data units = counts/sec. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 005 EU/mL to 5. Sale price $18,499. Set up the instrument and software. Test up to 3. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. For biochemical assays, the washer removes unbound material and unreacted reagents. Please click on the links below to view the Microplate Reader Comparison Tables. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. +44-118-944-8000. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Sunnyvale, Calif. Description Figure 1. These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. GxP regulated industry assessments of microplate readers. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Shortside barcode reader: 0310-5102. 40266 Email: drugdiscoverytech@lifetech. 9000-0756. The plate was incubated at room temperature for 50 minutes or 2. The plate is set up for reading and images are acquired according to specified parameters. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. . 58×38×22 cm (22. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The absorbance (A405) can be measured by the plate reader and is proportional to the endotoxin concentration in the sample. Tests every subsystem of the reader or . The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. 1. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Multimode microplate readers offer flexibility, and ease of use. visible absorbance, fluorescence. Select Update Driver. The computer controlling the plate reader runs Windows XP. The plate is set up for reading and images are acquired according to specified parameters. Microplate Stacker and Washer. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Research-grade results. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Shop now. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Molecular Devices, LLC. One plate carrier adapter is provided with the instrument. Supplier: Molecular Devices. Turn the reader on and secure the USB cable. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplateMicroplate Spectrophotometers. Step 1: Capture antibody binds to ELISA plate wells. Molecular Devices Flourmeteric Imaging. Step 4: Incubate at room temperature for 1 hour, protected from light. Endpoint. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. One plate carrier adapter is provided with the instrument. 4 °C above ambient to 45 °C. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 9. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Longside barcode reader: 0310-5101. HTRF-compatible SpectraMax Multi-Mode Readers. 997 (n = 3). 0 . 2. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Standard features include a cuvette port,. It may be named "Pothos". Step. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0) OD (0. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. BioTek Synergy H1 Hybrid OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader. Molecular Devices technical support is available by phone and e-mail. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Measure more accurately. Fluorescence Microplate Reader Comparison Chart. 4: Test tube cover. Catalog Number: (89212-396) Supplier: Molecular Devices. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Experimental details and customization of the group table. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. This process should take about 3 - 5 minutes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPrimary considerations when choosing a microplate reader are throughput and flexibility. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. #4TI-0214) UltraVision Plate 96 (Brooks Life. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 1-1. 6 % of reading 4. Supersedes 1. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Versa. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 020 OD 2. To contact technical support by phone in the U. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. NORTH AMERICA. Please contact your Molecular Devices representative for configuration options. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. to effectively test for endotoxins in samples. 00 USD. 00. Monday to Friday 8am - 5pm GMT. The reader measures both flat and round 96-well microplates and. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The most published microplate reader control and data analysis software. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The system provides automation for up to 50 microplates for easy walkaway automation. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 2. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Videos & Webinars. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Double-click the unknown device with the yellow warning icon. FlexStation® 3 Multi-Mode Microplate Reader. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. If the computer is connected to the Internet: Select Search Automatically for. The sample is typically placed in a cuvette through which light is sent horizontally. and fluorescence microplate readers—. Wait 20 seconds. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The former have been the traditional domain of plate readers and great strides have been. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Lamp source is a Xenon flash lamp (1 Joule/flash). 40-plate magazine (pair) 0200-6017. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. This reader's dual monochromators for. Molecular Devices, LLC. Microplate Readers. Your applications, your modes, your choice. Multi-mode Plate Reader. Molecular Devices. Instrument settings can be saved as a protocol file. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Laboratory automation workflows can help. Compact designs. Cells in each. 5: Schematic of. Versa Max. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. SoftMax Pro Software. SoftMax Pro 7. Plus 384. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. To update the driver. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. The plate is set up for reading and images are acquired accordingSpectraMax Readers have the most complete level of product validation and compliance. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. tools to expand the boundaries of their research. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 × 107) RLU 0. One plate carrier adapter is provided with the instrument. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The SpectraMax M2 and M2e are versatile and reliable. 1. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. Also see: SoftMax® Pro Microplate Software User Manual Download Page. . 1. 0. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. For more sample throughput, the system can be easily integrated into a full robotic to that of dedicated single-mode readers. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 9. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. 306 plate air cal. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Molecular Devices SpectraMax Gemini XS microplate reader with laptop and software (Pre-owned) $5,500. Supersedes 1. Scanning options are endpoint, kinetic, spectral. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. QuickSync. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. #6590-0118) Filter 665/10 (Molecular Devices cat. Incubate at RT. With SoftMax Pro Software, the PathCheck Sensor automatically normalizesThe SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. These systems are hybrids that allow you to choose between. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. 96-well plate and 12,500-20,000. Weight. The instrument supports the following read modes:. Gemini EM is a microplate spectrofluorometer from Molecular Devices. Important things to consider when purchasing a multimode microplate reader. The content of this site is published by the site. For nearly 30 years, Molecular Devices has provided scientists with . ORDER. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 18 kg. Lab automation for high-throughput plate-based assays. 1. V. 0) OD (500 to 1100) RFU (5. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. In an analogous manner, the same sample names were assigned to a CuvetteSet section. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. One plate carrier adapter is provided with the instrument. Company Molecular Devices, LLC. D. Lift up the lid over the chamber to insert or remove a cuvette. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The Back Panel Figure 1. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. The advantage of using labeled probes is detection specificity. #6590-0118) Filter 665/10 (Molecular Devices cat. 13. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. FLIPR Calcium 4 Assay Kit. A control well was set up to verify the accuracy of the standard curve. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. SpectraDrop High-Throughput Screening (HTS) Kit. Microplate Reader Shelf. 8×15×8. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 2 Software Release Notes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This offer includes SpectraMax i3x, SpectraMax. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Flexibility to read a variety of plate formats. please click here. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. Step 1: Turn on your microplate reader. Molecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. M2. Turn on Plate Reader and wait for it to complete its start-up routine. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax Microplate Readers. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. DOCUMENTATION. Clone Screening. No accessories are needed and SoftMax® Pro Software. Select Start>Control panel>Device Manager. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. Shop now. SoftMax Pro 7. Under "Category", select "Plate Type". United States Phone: 800-635-5577 408-747-1700. If it looks fine, then proceed. Absorbance Plate Reader Comparison Chart. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. . StakMax Handling Stacker Base Unit. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Increase productivity with patented microplate reader technologies. Molecular Devices Software. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. • 0. The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. 5 µL of each sample was pipetted into a 96-well. High-precision 9 channel system; filter-less with up to 6 wavelengths. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Sale price $3,495. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Instrument settings can be saved as a protocol file. . plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Select the Driver tab. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below.